n. 多重退火循环扩增法(Multiple Annealing and Looping Based Amplification Cycles)的缩写。是一种准线性的全基因组扩增方法,利用特殊引物使扩增子具有互补末端以形成环状,防止DNA被指数复制,从而减少扩增偏差。MALBAC常用于鸟枪法生成有重叠、涵盖大部分基因组的扩增子,并在完成后进行常规PCR进一步扩增以进行下一代测序
· MALBAC is a quasi-linear whole genome amplification method that uses special primers to create complementary ends in the amplified DNA fragments, preventing exponential replication and reducing amplification bias.